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Translational Bio-Nanosciences Laboratory: Dmitri Simberg, PhD
Research
We value random discovery, serendipity, and power of observation. At the same time, we pursue systematic studies using a variety of methodologies, from serum biochemistry, to in vivo pharmacology to population studies in healthy persons and in patients. We are always looking for enthusiastic and energetic individuals.
Special Projects
Intravenously injected nanomaterials undergo interactions with plasma components and immune receptors, with subsequent clearance by body macrophages and monocytes. This process is the major problem in nanomedicine since it decreases the dose of nanoparticles in the tumor, limits the efficiency of imaging and therapy, and causes toxicity. There is still insufficient knowledge of the basic mechanisms that lead to nanoparticle recognition and elimination.
Intravenously injected nanomaterials undergo interactions with plasma components and immune receptors, with subsequent clearance by body macrophages and monocytes. This process is the major problem in nanomedicine since it decreases the dose of nanoparticles in the tumor, limits the efficiency of imaging and therapy, and causes toxicity. There is still insufficient knowledge of the basic mechanisms that lead to na
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Published in final edited form as: Adv Nanobiomed Res. 2022 Jul 19;2(8):2200037. doi: 10.1002/anbr.202200037
Abstract
Red blood cells (RBCs) are natural carriers for sustained drug delivery, imaging, and in vivo sensing. One of the popular approaches to functionalize RBCs is through lipophilic anchors, but the structural requirements for anchor stability and in vivo longevity remain to be investigated. Using fluorescent lipids with the same cyanine 3 (Cy3) headgroup but different lipid chain and linker, the labeling efficiency of RBCs and in vivo stability are investigated. Short-chain derivatives exhibited better insertion efficiency, and mouse RBCs are better labeled than human RBCs. Short-chain derivatives demonstrate low retention in vivo. Derivatives with ester bonds are especially unstable, due to removal and degradation. On the other hand, long-chain, covalently linked derivatives show remarkably long retention and stability (over 80 days half life in the membrane). The clearance organs are liver and spleen with evidence of lipid transfer to the liver sinusoidal endothelium. Notably, RBCs modified with PEGylated lipid show decreased macrophage uptake. Some of the derivatives promote binding of antibodies in human p